cyclin d1 (Cell Signaling Technology Inc)
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Cyclin D1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 467 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+anti+cyclin+d1/Cyclin+A2+Mouse+mAb/pm41811891-92-60-62
Average 96 stars, based on 467 article reviews
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Blocking Assay:Article Title: Protein kinase C epsilon activation regulates proliferation, migration, and epithelial to mesenchymal-like transition in rat Schwann cells Article Snippet: An equal amount of proteins (20–30 μg) was separated through SDS-PAGE electrophoresis and transferred to a nitrocellulose membrane (GE Healthcare, Milan, Italy). .. After blocking, the membranes were exposed to primary antibodies: Western Blot:Article Title: β-elemene alleviates airway stenosis via the ILK/Akt pathway modulated by MIR143HG sponging miR-1275 Article Snippet: .. Primary antibodies used for Western blotting of the tissue protein were: Mouse anti-ILK (1:500, Santa Cruz Biotechnology, Cat# sc-20019 RRID: AB_627807), Mouse anti-Akt (1:1000, Cell Signaling Technology, Cat#2920 RRID: AB_1147620), Mouse anti-Phospho-Akt (Ser473) (1:1000, Cell Signaling Technology, Cat# 4051 RRID: AB_331158), Article Title: Cdk2 catalytic activity is essential for meiotic cell division in vivo Article Snippet: Blots were probed with the indicated primary antibodies overnight at 4°C, followed by secondary goat antimouse (Pierce, 0031432) or antirabbit antibodies (Pierce, 0031462) conjugated with horseradish peroxidase, and were developed using enhanced chemiluminescence (PerkinElmer, NEL105001EA). .. All antibodies used for immunoblotting were obtained from commercial suppliers: mouse anti-Cdk1 (Santa Cruz, sc-54), mouse anti-Cdk2 (Santa Cruz, sc-6248), mouse anti-Cdk4 (Cell Signaling, #2906), rabbit anti-cyclin A2 (Santa Cruz, sc-596), mouse anti-cyclin A2 (Biosource, AHF0022), mouse anti-cyclin B1 (Cell Signaling, #4135), Immunohistochemistry:Article Title: Targeting Cyclin D-CDK4/6 Sensitizes Immune-Refractory Cancer by Blocking the SCP3–NANOG Axis Article Snippet: .. For IHC, we used the following primary antibodies: rabbit anti-phopho-akt T308 (1:250, Cell Signaling Technology) and Immunohistochemical staining:Article Title: β-elemene alleviates airway stenosis via the ILK/Akt pathway modulated by MIR143HG sponging miR-1275 Article Snippet: Images were finally obtained under an optical microscope (Eclipse Ti2, Nikon). .. Primary antibodies used for immunohistochemical staining were as listed below: Mouse anti-ILK (1:100, Santa Cruz Biotechnology, Cat# sc-20019, RRID:AB_627807), Mouse anti-Akt (1:200, Cell Signaling Technology, Cat#2920, RRID:AB_1147620), Mouse anti-Phospho-Akt (Ser473) (1:100, Cell Signaling Technology, Cat# 4051, RRID:AB_331158), Staining:Article Title: β-elemene alleviates airway stenosis via the ILK/Akt pathway modulated by MIR143HG sponging miR-1275 Article Snippet: Images were finally obtained under an optical microscope (Eclipse Ti2, Nikon). .. Primary antibodies used for immunohistochemical staining were as listed below: Mouse anti-ILK (1:100, Santa Cruz Biotechnology, Cat# sc-20019, RRID:AB_627807), Mouse anti-Akt (1:200, Cell Signaling Technology, Cat#2920, RRID:AB_1147620), Mouse anti-Phospho-Akt (Ser473) (1:100, Cell Signaling Technology, Cat# 4051, RRID:AB_331158), Construct:Article Title: JAB1/COPS5 is a putative oncogene that controls critical oncoproteins deregulated in prostate cancer. Article Snippet: Recent evidence support that the c-Jun activation domain-binding protein 1 (JAB1)/COPS5 has an oncogenic function in various tissues.. We show that JAB1 amplification in human prostate cancer (PCa) correlates with reduced overall survival and disease-free progression.. Immunohistochemical staining shows enhanced expression of JAB1 in the cytoplasmic compartment of PCa cells compared to the normal prostate epithelium, indicating the activity/function of JAB1 is altered in PCa. Incubation:Article Title: The effect of lithium and lithium-loaded hyaluronic acid hydrogel applications on nerve regeneration and recovery of motor functions in peripheral nerve injury Article Snippet: Functional recovery rates after peripheral nerve injury are quite poor.. Studies have focused on the organization of axonal development and the alteration of the microenvironment of the regenerated nerve.. It is a frequently used repair method to place a tube to the incision area with tubulization technique and to try increasing the effect by filling the tube with various substances. Immunofluorescence:Article Title: The effect of lithium and lithium-loaded hyaluronic acid hydrogel applications on nerve regeneration and recovery of motor functions in peripheral nerve injury Article Snippet: Functional recovery rates after peripheral nerve injury are quite poor.. Studies have focused on the organization of axonal development and the alteration of the microenvironment of the regenerated nerve.. It is a frequently used repair method to place a tube to the incision area with tubulization technique and to try increasing the effect by filling the tube with various substances. |
