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cyclin d1  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc cyclin d1
    Cyclin D1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 467 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+anti+cyclin+d1/Cyclin+A2+Mouse+mAb/pm41811891-92-60-62
    Average 96 stars, based on 467 article reviews
    cyclin d1 - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Blocking Assay:

    Article Title: Protein kinase C epsilon activation regulates proliferation, migration, and epithelial to mesenchymal-like transition in rat Schwann cells
    Article Snippet: An equal amount of proteins (20–30 μg) was separated through SDS-PAGE electrophoresis and transferred to a nitrocellulose membrane (GE Healthcare, Milan, Italy). .. After blocking, the membranes were exposed to primary antibodies: mouse anti-cyclin D1 (1:1,000; #2926, Cell Signaling, Rehovot, Israel), mouse anti-CDK6 (1:1,000; #3136, Cell Signaling), rabbit anti-Snail (1:1,000; #3879, Cell Signaling), rabbit anti-vimentin (1:1,000; #5741, Cell Signaling), mouse anti-E-cadherin (1:1,000; sc-8426, Santa Cruz Biotechnology, Dallas, TX, USA), mouse anti-N-cadherin (1:1,000; sc-59987, Santa Cruz Biotechnology), mouse anti-alpha-tubulin (1:2,000; T6199, Merk Life Science), and rabbit anti-P0 (1:500; 10572-1-AP, Proteintech, Manchester, UK). .. Then, the membranes were incubated with HRP-conjugated anti-rabbit or anti-mouse secondary antibody for 1 h at room temperature and revealed using an enhanced chemiluminescence kit Cyanagen Ultra (Cyanagen, Bologna, Italy).

    Western Blot:

    Article Title: β-elemene alleviates airway stenosis via the ILK/Akt pathway modulated by MIR143HG sponging miR-1275
    Article Snippet: .. Primary antibodies used for Western blotting of the tissue protein were: Mouse anti-ILK (1:500, Santa Cruz Biotechnology, Cat# sc-20019 RRID: AB_627807), Mouse anti-Akt (1:1000, Cell Signaling Technology, Cat#2920 RRID: AB_1147620), Mouse anti-Phospho-Akt (Ser473) (1:1000, Cell Signaling Technology, Cat# 4051 RRID: AB_331158), Mouse anti-cyclin D1 (1:1000, Cell Signaling Technology, Cat# 2906 RRID: AB_2070400), Mouse anti-Bcl-2 (1:1000, Cell Signaling Technology, Cat#15071 RRID: AB_2744528). .. The secondary antibody used was HRP-conjugated goat anti-Mouse IgG (1:2000, Abcam, cat# ab97040, RRID: AB_10698223).

    Article Title: Cdk2 catalytic activity is essential for meiotic cell division in vivo
    Article Snippet: Blots were probed with the indicated primary antibodies overnight at 4°C, followed by secondary goat antimouse (Pierce, 0031432) or antirabbit antibodies (Pierce, 0031462) conjugated with horseradish peroxidase, and were developed using enhanced chemiluminescence (PerkinElmer, NEL105001EA). .. All antibodies used for immunoblotting were obtained from commercial suppliers: mouse anti-Cdk1 (Santa Cruz, sc-54), mouse anti-Cdk2 (Santa Cruz, sc-6248), mouse anti-Cdk4 (Cell Signaling, #2906), rabbit anti-cyclin A2 (Santa Cruz, sc-596), mouse anti-cyclin A2 (Biosource, AHF0022), mouse anti-cyclin B1 (Cell Signaling, #4135), mouse anti-cyclin D1 (Cell Signaling, #2926), rabbit anti-cyclin E1 (eBioscience, 146714), mouse anti-p21 (Santa Cruz, sc-6246), mouse anti-p27 (BD Transduction, 610242), mouse anti-Cdc6 (Santa Cruz, sc-9964), rabbit anti-MCM2 (Cell Signaling, #3619), rabbit anti-Stat1 (Cell Signaling, #9172), rabbit anti-Stat3 (Cell Signaling, #9132), mouse anti-α-tubulin (Sigma, F2168), mouse anti-γ-tubulin (Sigma, T6557), mouse anti-Hsp90 (BD Transduction, 610419), and goat anti-actin (Santa Cruz, sc-1616). ..

    Immunohistochemistry:

    Article Title: Targeting Cyclin D-CDK4/6 Sensitizes Immune-Refractory Cancer by Blocking the SCP3–NANOG Axis
    Article Snippet: .. For IHC, we used the following primary antibodies: rabbit anti-phopho-akt T308 (1:250, Cell Signaling Technology) and mouse anti-cyclin D1 (1:200, Cell Signaling Technology). .. For fluorescent detection of antibody labeling, we used Alexa Fluor 568 goat anti-rabbit, anti-mouse (1:500, Molecular Probes).

    Immunohistochemical staining:

    Article Title: β-elemene alleviates airway stenosis via the ILK/Akt pathway modulated by MIR143HG sponging miR-1275
    Article Snippet: Images were finally obtained under an optical microscope (Eclipse Ti2, Nikon). .. Primary antibodies used for immunohistochemical staining were as listed below: Mouse anti-ILK (1:100, Santa Cruz Biotechnology, Cat# sc-20019, RRID:AB_627807), Mouse anti-Akt (1:200, Cell Signaling Technology, Cat#2920, RRID:AB_1147620), Mouse anti-Phospho-Akt (Ser473) (1:100, Cell Signaling Technology, Cat# 4051, RRID:AB_331158), Mouse anti-cyclin D1 (1:500, Cell Signaling Technology, Cat# 2926, RRID:AB_2070400), Mouse anti-Bcl-2 (1:250, Cell Signaling Technology, Cat#15071, RRID:AB_2744528), Mouser anti-ki67 (1:100, Nevus, Cat# nbp2-22112). .. The HRP-conjugated secondary antibody used was HRP-conjugated goat anti-Mouse IgG (1:5000, Abcam, Cat# ab97040, RRID: AB_10698223).

    Staining:

    Article Title: β-elemene alleviates airway stenosis via the ILK/Akt pathway modulated by MIR143HG sponging miR-1275
    Article Snippet: Images were finally obtained under an optical microscope (Eclipse Ti2, Nikon). .. Primary antibodies used for immunohistochemical staining were as listed below: Mouse anti-ILK (1:100, Santa Cruz Biotechnology, Cat# sc-20019, RRID:AB_627807), Mouse anti-Akt (1:200, Cell Signaling Technology, Cat#2920, RRID:AB_1147620), Mouse anti-Phospho-Akt (Ser473) (1:100, Cell Signaling Technology, Cat# 4051, RRID:AB_331158), Mouse anti-cyclin D1 (1:500, Cell Signaling Technology, Cat# 2926, RRID:AB_2070400), Mouse anti-Bcl-2 (1:250, Cell Signaling Technology, Cat#15071, RRID:AB_2744528), Mouser anti-ki67 (1:100, Nevus, Cat# nbp2-22112). .. The HRP-conjugated secondary antibody used was HRP-conjugated goat anti-Mouse IgG (1:5000, Abcam, Cat# ab97040, RRID: AB_10698223).

    Construct:

    Article Title: JAB1/COPS5 is a putative oncogene that controls critical oncoproteins deregulated in prostate cancer.
    Article Snippet: Recent evidence support that the c-Jun activation domain-binding protein 1 (JAB1)/COPS5 has an oncogenic function in various tissues.. We show that JAB1 amplification in human prostate cancer (PCa) correlates with reduced overall survival and disease-free progression.. Immunohistochemical staining shows enhanced expression of JAB1 in the cytoplasmic compartment of PCa cells compared to the normal prostate epithelium, indicating the activity/function of JAB1 is altered in PCa.

    Incubation:

    Article Title: The effect of lithium and lithium-loaded hyaluronic acid hydrogel applications on nerve regeneration and recovery of motor functions in peripheral nerve injury
    Article Snippet: Functional recovery rates after peripheral nerve injury are quite poor.. Studies have focused on the organization of axonal development and the alteration of the microenvironment of the regenerated nerve.. It is a frequently used repair method to place a tube to the incision area with tubulization technique and to try increasing the effect by filling the tube with various substances.

    Immunofluorescence:

    Article Title: The effect of lithium and lithium-loaded hyaluronic acid hydrogel applications on nerve regeneration and recovery of motor functions in peripheral nerve injury
    Article Snippet: Functional recovery rates after peripheral nerve injury are quite poor.. Studies have focused on the organization of axonal development and the alteration of the microenvironment of the regenerated nerve.. It is a frequently used repair method to place a tube to the incision area with tubulization technique and to try increasing the effect by filling the tube with various substances.



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    The endoscopic presentation and pathological features of gastric LCH A : Endoscopic examination revealed a small polyp in the gastric body, measuring 0.5 cm in diameter. B : The tumor cells are located in the mucosal layer and show clear boundaries with the surrounding tissue (H&E, 200×). C : The tumor cells exhibit round or oval nuclei, with some cells showing nuclear grooves. Eosinophils are scattered in the background, and occasional mitotic figures are observed (H&E, 400×). D : Diffuse expression of Langerin (EnVision method, 400×). E : Diffuse expression of CD1a (EnVision method, 400×). F : Positive expression of <t>Cyclin</t> <t>D1</t> (EnVision method, 400×). G : Positive expression of S-100 (EnVision method, 400×). H : Positive expression of Ki-67 (EnVision method, 200×).
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    Image Search Results


    The endoscopic presentation and pathological features of gastric LCH A : Endoscopic examination revealed a small polyp in the gastric body, measuring 0.5 cm in diameter. B : The tumor cells are located in the mucosal layer and show clear boundaries with the surrounding tissue (H&E, 200×). C : The tumor cells exhibit round or oval nuclei, with some cells showing nuclear grooves. Eosinophils are scattered in the background, and occasional mitotic figures are observed (H&E, 400×). D : Diffuse expression of Langerin (EnVision method, 400×). E : Diffuse expression of CD1a (EnVision method, 400×). F : Positive expression of Cyclin D1 (EnVision method, 400×). G : Positive expression of S-100 (EnVision method, 400×). H : Positive expression of Ki-67 (EnVision method, 200×).

    Journal: Journal of Clinical and Experimental Hematopathology : JCEH

    Article Title: Clinicopathological features of gastric Langerhans cell histiocytosis and a literature review

    doi: 10.3960/jslrt.25082

    Figure Lengend Snippet: The endoscopic presentation and pathological features of gastric LCH A : Endoscopic examination revealed a small polyp in the gastric body, measuring 0.5 cm in diameter. B : The tumor cells are located in the mucosal layer and show clear boundaries with the surrounding tissue (H&E, 200×). C : The tumor cells exhibit round or oval nuclei, with some cells showing nuclear grooves. Eosinophils are scattered in the background, and occasional mitotic figures are observed (H&E, 400×). D : Diffuse expression of Langerin (EnVision method, 400×). E : Diffuse expression of CD1a (EnVision method, 400×). F : Positive expression of Cyclin D1 (EnVision method, 400×). G : Positive expression of S-100 (EnVision method, 400×). H : Positive expression of Ki-67 (EnVision method, 200×).

    Article Snippet: The primary antibodies used included S-100 (mouse monoclonal, clone 4C4.9, 1:150; Zhongshan Golden Bridge Biotechnoloy Co., Ltd., Beijing, China), Langerin (mouse monoclonal, clone 12D6, Zhongshan Golden Bridge Biotechnology Co., Ltd., Beijing, China), Cyclin D1 (mouse monoclonal, clone SA38‐08, Zhongshan Golden Bridge Co., Ltd., Beijing, China), CD68 (mouse monoclonal, clone KP-1, 1:200; Maixin Biotechnology Development Co., Ltd., Beijing, Fuzhou, China), CD1a (mouse monoclonal, clone O10, 1:100; Maixin Biotechnology Development Co., Ltd., Beijing, Fuzhou, China), CD20 (mouse monoclonal, clone L26, 1:100; Maixin Biotechnology Development Co., Ltd., Beijing, Fuzhou, China), AE1/AE3 (mouse monoclonal, clone AE1/3, 1:200; Gene Technology Co., Ltd., Shanghai, China), Ki‐67 (mouse monoclonal, clone GM027, 1:200; Gene Technology Co., Ltd., Shanghai, China), and CD3 (mouse monoclonal, clone LN10, 1:100; Gene Technology Co., Ltd., Shanghai, China).

    Techniques: Expressing